What is trnL intron?

What is trnL intron?

The chloroplast trnL (UAA) intron may represent a good target region for our purpose. Its sequences have been widely used for reconstructing phylogenies between closely related species (13–15) or for identifying plant species (16,17).

What is trnL?

The trnL gene is part of trnL-F region of chloroplast genome that split by group I intron, the intergenic spacer and trnF exons (Figure 1) and is co-transcribed (Bakker et al. 2000). The intron is positioned between the U and the A of the UAA anticodon loop.

What is trnL UAA?

The trnL(UAA) intron interrupts the anticodon loop of the tRNALeu, which is encoded in the large single copy region of the plastid genome. In the chloroplast DNA, trnL is the only Group I intron region having conserved secondary structure [7,8] with alternation of conserved and variable regions [9].

What is rbcL marker?

The rbcL marker based method was adopted to identify green isolates from monospecies but non-axenic cultures of microalgae with 18S universal markers as control. In present study, authors have developed and validated a novel method for molecular identification of microalgae even from non- axenic cultures.

How many amino acids are in matK?

sativum had percent identity with C. cajan greater than 80% (Tables 2 & 3). Physicochemical properties of matK protein showed that the number of amino acid residues ranged from 199-509 with G.

What does rbcL do in plants?

The chloroplast gene rbcL encodes the large subunit of ribulose bisphosphate carboxylase. In Chlamydomonas reinhardtii, this gene is transcribed more actively than any other protein-encoding chloroplast gene studied to date.

Why is rbcL used?

The rbcL gene is a useful barcode because it codes for part of the key photosynthesis enzyme ribulose bisphosphate carboxylase (RuBisCo), so it is present in virtually all plant species. One section of its DNA sequence is very variable between species, making it ideal for DNA barcoding.

How do you make a DNA barcode?

The Process

  1. Step 1: Isolate DNA from the sample.
  2. Step 2: Amplify the target DNA barcode region using PCR.
  3. Step 3: Sequence the PCR products.
  4. Step 4: Compare the resulting sequences against reference databases to find the matching species.

Where is MatK gene found?

The matK gene of chloroplast is 1500 bp long, located within the intron of the trnK and codes for maturase like protein, which is involved in Group II intron splicing.

What is the function of MatK?

Maturase K (matK) is a plant plastidial gene. The protein it encodes is an organelle intron maturase, a protein that splices Group II introns. It is essential for in vivo splicing of Group II introns.