How big is SUMO protease?
The free 6xHis-SUMO tag has an expected molecular weight of 12 kDa, but migrates at ~15-18 kDa. If partial cleavage is observed, another aliquot of SUMO Express Protease may be added and digestion continued at 4 °C overnight.
Why is SUMO tag used?
SUMO Tag Definition Sumo tag is most frequently used as N-end fusion sequence in yeast to increase the expression and solubility of the desired recombinant protein. SUMO proteins are similar to ubiquitin in their folded structure but possess only about 20% homology to the amino acid sequence of ubiquitin.
How do you get rid of protease?
Non-tagged proteases such as thrombin or Factor Xa can be removed using a Benzamidine resin, which binds to serine proteases. Tagged proteases can be removed with the same affinity chromatography resin/column used to capture the tagged protein of interest.
What is ULP protease?
SUMO Protease, also known as Ulp, is a recombinant fragment of ULP1 (Ubl-specific protease 1) from Saccharomyces cerevisia e. It is highly specific for the SUMO protein fusion, recognizing the tertiary structure of SUMO rather than an amino acid sequence.
What does ULP stand for?
SUMO protease, also known as Ulp, is a recombinant fragment of ULP1 (Ubl-specific protease 1) from Saccharomyces cerevisiae. It is highly specific for the SUMO protein fusion, recognizing the tertiary structure of SUMO rather than an amino acid sequence.
What is the role of SUMO protease Ulp1 in pore integrity?
Mutations or physiological perturbations altering nuclear pore integrity decrease the levels of the nuclear pore complex (NPC)-associated SUMO protease Ulp1 and trigger the accumulation of sumoylated versions of Hek2 unable to bind NPC mRNAs.
What is the role of Ulp1 in telomere silencing?
Moderate elevation of Ulp1 reduces silencing of both, the euchromatic gene placed proximal to telomeric repeats and the sub-telomeric genes that are silenced by TPE.