What is in minimum essential media?

What is in minimum essential media?

Eagle’s minimum essential medium modified to contain Earle’s Balanced Salt Solution, non-essential amino acids, 2 mM L-glutamine, 1 mM sodium pyruvate, and 1500 mg/L sodium bicarbonate.

What is the difference between Emem and MEM?

EMEM, 2X contains 2 times the amount of MEM, 4 times the vitamins and amino acids of the original MEM, and Earle’s salts which allows for the culturing of an even wider variety of more nutritionally fastidious cells.

How do you prepare minimum essential medium?

Product Description :

  1. Minimum Essential Medium (MEM) is a modification of Basal Medium Eagle (BME).
  2. Calcium chloride dihydrate.
  3. Glycine.
  4. Choline bitartarate.
  5. D-Glucose.
  6. Suspend 10.6gms of the powder in 900ml tissue culture grade water with constant, gentle stirring until the powder is completely dissolved.

What is MEM in cell culture?

Minimum Essential Medium (MEM) was developed by Harry Eagle and is one of the most widely used medias of all synthetic cell culture media. MEM is simply a modified Basal Medium Eagle media containing higher concentrations of essential nutrients.

Can I use DMEM instead of MEM?

DMEM is richer in a variety of nutrients than MEM and is a very good choice for many cell types. Cells will grow faster and deplete the medium slower in DMEM. You should also consider Opti-MEM which, in my experience, is even better than DMEM.

Why is FBS used in cell culture?

The primary use of FBS is a supplement for in vitro cell culture. Its unique biological makeup promotes rapid cell growth, thus making it a product that yields a high efficacy.

What are Earle’s salts?

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  • Earle’s Balanced Salt Solution (EBSS) is an isotonic buffer solution containing inorganic salts and a carbohydrate as an energy source.
  • EBSS is supplied in gamma-irradiated, sterile PETG or PETE bottles.

What percentage of serum is used in culture medium?

Normal growth media often contain 2-10% of serum. Supplementation of media with serum serves the following functions [36] : Serum provides the basic nutrients (both in the solution as well as bound to the proteins) for cells.

What is RPMI used for?

RPMI is used when laboratory testing requires fresh, unfixed specimens such as flow cytometry as well as some FISH, molecular and cytogenetics tests. 2 ) Why is it pink/red? It has a phenol red pH indicator that will turn from pink to yellow indicating contamination or exposure to air.

What is modified Eagle’s medium?

DMEM (Dulbecco’s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.

Can I use DMEM instead of EMEM?

The main difference between DMEM and EMEM is that DMEM contains four times more vitamins and amino acids and two to four times more in comparison to the EMEM formula whereas EMEM is based on six salts and glucose. Furthermore, DMEM contains iron in the form of ferric sulfate.

Is RPMI serum free?

RPMI 1640 has traditionally been used for the serum-free expansion of human lymphoid cells. RPMI 1640 uses a bicarbonate buffering system and differs from most mammalian cell culture media in its typical pH 8 formulation.

Why choose GIBCO cell culture media?

Time-tested and trusted, our Gibco cell culture media line includes products designed to support the growth and maintenance of a variety of mammalian cells and cell lines. We’ve developed ready-to-use cell culture media products as well as powdered and concentrated liquid formulations to fit your experimental setup and your budget.

What is GIBCO Mem (human plasma-like medium)?

Gibco Human Plasma-like Medium (HPLM) is a new formulation designed to resemble the natural cellular environment found in the body, mimicking the metabolic profile of human plasma. Select the right Gibco MEM for your cell culture. Browse the catalog and you’ll be able to narrow the list according to:


Gibco Human Plasma-like Medium (HPLM) is a new formulation designed to resemble the natural cellular environment found in the body, mimicking the metabolic profile of human plasma. Select the right Gibco MEM for your cell culture.

What are the benefits of GIBCO bottles?

The 1 L Gibco bottle uses 38% less source material (32% less for the 0.5 L bottle) than comparable media bottles on the market today. This reduces the consumption of non-renewable resources helping shrink your footprint.