What is an IDT G block?
gBlocks™ Gene Fragments are double-stranded DNA fragments of 125–3000 bp in length. They are the industry standard for double-stranded gene fragments, designed for affordable and easy gene construction or modification, applications such as antibody research and CRISPR-mediated genome editing, qPCR standards, and more.
How do you resuspend a gene block?
Resuspending gBlocks Gene Fragments
- Before opening the tube, spin it down in a microcentrifuge for 3–5 seconds to ensure the DNA is in the bottom of the tube.
- Add molecular grade water, or a buffer such as IDTE, to reach a final concentration of 10 ng/µL.
- Vortex briefly.
- Incubate at approximately 50°C for 15–20 min.
Can you order double stranded DNA?
Yes, IDT can synthesize duplex oligos. When entering your oligo, select the Duplex option on the order form. Selecting this option will load a revised order form with space for the sequence of a complementary strand.
How do you amplify Gblock?
If you must amplify your gBlocks fragment, use the minimum amount of starting template required by your PCR kit protocol, limit the cycle number to 10–12, and use a high-fidelity polymerase.
Are gBlocks methylated?
gBlocks Gene Fragments and PrimeTime qPCR Assays support simple methylation analysis method.
What are gene fragments?
Gene fragments are pieces of genes containing only the exons (those parts of the gene which actually encode the protein sequence).
Are oligonucleotides double stranded?
Oligonucleotides or oligos are short single-stranded or double-stranded polymers of nucleic acids.
Can viruses have double stranded DNA?
Any of the viruses belonging to the Class I of Baltimore classification system characterized by having a double stranded DNA as the genetic material and using a DNA-dependent DNA polymerase during replication. Most of these viruses must enter the host nucleus before they can replicate.
How does methylation analysis work?
Methylation analysis is the study of chromosomal patterns of DNA or histone modification by methyl groups. The cytosine (C) base in DNA and lysine residue in histone tails can be methylated.
What causes DNA fragmentation?
What caused the DNA to become fragmented? The restriction enzyme.
How do I contact IDT for help with my GBlocks gene fragments design?
If you have any questions or need further assistance with your gBlocks Gene Fragments design, please contact an IDT Synthetic Biology Product Specialist at [email protected]. gBlocks ® Gene Fragments may contain standard DNA bases (A,C,G,T). Numbers and spaces in the sequence will be ignored.
What are Gblocks™ gene fragments?
gBlocks™ Gene Fragments are double-stranded DNA fragments of 125–3000 bp in length. They are the industry standard for double-stranded gene fragments, designed for affordable and easy gene construction or modification, applications such as antibody research and CRISPR-mediated genome editing, qPCR standards, and more.
Why IDT custom DNA oligos?
Custom DNA oligos IDT’s long-standing reputation as a pioneer and leader in custom oligo manufacturing is primarily due to our proprietary synthesis platforms.
What is the error rate of IDT gene fragments?
With an industry leading error rate of up to 1:12,000 for gBlocks HiFi Gene Fragments, IDT Gene Fragments demonstrate a high probability of first-time cloning success.