What is Phusion polymerase?
Phusion Polymerase: robust and accurate high-fidelity PCR The enzyme, originally developed by Finnzymes, is a fusion of a proofreading polymerase and a processivity-enhancing domain, creating one of the most reliable and robust polymerases available.
How is Klenow fragment different from DNA pol?
The key difference between Klenow fragment and DNA polymerase 1 is that Klenow fragment lacks 5′ to 3′ exonuclease activity while DNA polymerase 1 has 5′ to 3′ exonuclease activity. Therefore, Klenow fragment has two domains, while DNA polymerase 1 has all three domains.
How does Taq polymerase work?
Once primers are attached, the Taq polymerase takes its position on the strand to produce the new strands by adding the dNTPs. This leads to the production of new complementary DNA (cDNA) strands. The newly synthesized strands thus act as templates in the next cycle of PCR. After each cycle, the DNA doubles.
What is the activity of Taq DNA polymerase?
Overview of PCR. Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1,2,3) and a 5´ flap endonuclease activity (4,5). It is supplied with 10X ThermoPol Reaction Buffer, which contains a nonionic detergent to increase enzyme stability during longer incubations.
What are the guidelines for the use of Neb’s Taq DNA polymerase?
The following guidelines are provided to ensure successful PCR using NEB’s Taq DNA Polymerase. These guidelines cover routine PCR. Amplification of templates with high GC content, high secondary structure, low template concentrations, or amplicons greater than 5 kb may require further optimization.
What is hot start Taq DNA polymerase?
Learn More Product Information Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and an aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal cycling conditions, allowing reactions to be set up at room temperature.
What are the different types of Taq used in PCR?
Taq is available with different formats to accommodate a variety of PCR applications. For example, Taq with standard Taq Buffer is designed to support existing PCR platforms, while Hot Start Taq formulations ( NEB #M0495) are also available for flexibility during reaction setup and increased specificity.