What is shRNA miR?
PLATINUM Select MLP Retroviral microRNA-adapted short hairpin RNA (shRNA-mir) are next generation vector-based RNAi triggers generated using the proprietary shERWOOD algorithm developed in Dr. Gregory Hannon’s laboratory at Cold Spring Harbor Laboratory.
What is shRNAmir?
Developed in collaboration with Greg Hannon (CSHL) and Steve Elledge (Harvard) the shRNAmir libraries provide unique solutions for your diverse RNAi needs. Features include: • Unique microRNA-30 based design greatly. increases knockdown specificity and efficiency. • Pre-cloned into viral vectors.
What is the function of a miR?
miR-590 plays a role of oncogenes or tumor suppressor and regulates cell differentiation, proliferation, apoptosis, other physiological and pathological process, and thus becomes an important molecular marker for disease diagnosis, treatment and prognosis.
What is an RNAi vector?
Double-stranded RNA-mediated interference (RNAi) has recently emerged as a powerful reverse genetic tool to silence gene expression in multiple organisms including plants, Caenorhabditis elegans, and Drosophila.
What is the difference between siRNA and shRNA?
siRNA refers to a single-stranded RNA molecule produced by the cleavage and processing of double-stranded RNA while shRNA refers to a short sequence of RNA which makes a tight hairpin turn and can be used to silence gene expression. Thus, this is the main difference between siRNA and shRNA.
How many human microRNAs are there?
Human genome encodes approximately 2,600 mature microRNAs (miRBase v. 22) and, according to GENCODE data (v. 29), more than 200,000 of transcripts, including isoforms with slight variations.
How does a riboswitch control protein production?
In molecular biology, a riboswitch is a regulatory segment of a messenger RNA molecule that binds a small molecule, resulting in a change in production of the proteins encoded by the mRNA.
What is an RNAi construct?
RNA interference (RNAi) is a biological process where RNA molecules are used to inhibit gene expression. Typically, short RNA molecules are created that are complementary to endogenous mRNA and when introduced into cells, bind to the target mRNA.
Are shRNA (Mir) s suitable for RNAi-based therapy in hemoglobinopathies?
These findings have important implications in future design of shRNA (miR)s for RNAi-based therapy in hemoglobinopathies and other diseases requiring lineage-specific expression of gene silencing sequences. Carrier Proteins Cell Lineage / genetics*
Do we need to optimize shRNA sequences upon incorporation into a miRNA backbone?
Our results suggest the requirement for optimization of shRNA sequences upon incorporation into a miRNA backbone. These findings have important implications in future design of shRNA (miR)s for RNAi-based therapy in hemoglobinopathies and other diseases requiring lineage-specific expression of gene silencing sequences.
What is shRNA (pre-miRNA)?
This first generation of shRNAs (pre-miRNA-like shRNA) was characterized by the use of RNA polymerase III promoters (Pol III). This enabled the precise synthesis of transcripts that folded into pre-miRNA-like stem-loop structures [5].
How to eliminate the miRNA-like off-targeting in shRNA repression?
As miRNA-like repression only requires the base pairing of as few as 6 nt in position 2–7 (seed region) of guide strand, it is nearly impossible to eliminate the miRNA-like off-targeting completely. One strategy is to take advantage of mechanistic differences between on-targets and off-targets shRNA repression.