What is proximity ligation analysis?

What is proximity ligation analysis?

Proximity ligation assay (PLA) is a unique method in which single-stranded oligonucleotides are conjugated to affinity binders of proteins, followed by amplification of the signal by DNA polymerization and hybridization of complementary oligonucleotides labeled with fluorogenic or chromogenic readout.

Is PLA quantitative?

Combining Duolink® PLA with flow cytometry allows collection of quantitative data in a high throughput manner.

How does proximity extension Assay work?

Proximity Extension Assay (PEA) is a 96-plex immunoassay for high throughput detection of protein biomarkers in liquid samples. For each biomarker, a matched pair of antibodies linked to unique oligonucleotides (proximity probes) binds to the respective protein target.

What are PLA probes?

In situ PLA uses oligonucleotide-modified antibodies, referred to as PLA probes or proximity probes, to visualize target proteins. Upon proximal binding by pairs of PLA probes, the conjugated oligonucleotides template ligation of secondarily added oligonucleotide pairs to generate DNA circles.

How does a PLA work?

A programmable logic array (PLA) is a kind of programmable logic device used to implement combinational logic circuits. The PLA has a set of programmable AND gate planes, which link to a set of programmable OR gate planes, which can then be conditionally complemented to produce an output.

What is olink technology?

The unique technology behind our Olink® Explore platform and Olink® Target 96 and Target 48 panels enables high-throughput, multiplex immunoassays of proteins using minimal volumes of serum, plasma, or almost any other type of biological sample.

What are proximity ligation assays?

Proximity ligation assays are a group of protein detection techniques in which reagents with affinity for target proteins, typically antibodies, are coupled to short strands of DNA.

What is the difference between homogeneous and solid phase proximity ligand (PLA)?

The added advantage of homogenous PLA is that solid support is not required to immobilize the target protein. Solid Phase Proximity Ligation Assay is an alternative methodology to homogenous PLA and utilizes a capture antibody to immobilize a target protein onto a solid phase, providing some advantages such as investigation of larger sample sets.

What is homogeneous proximity ligation?

Homogenous Proximity Ligation Assay is suitable for the quantitation of low concentrations of protein molecules in a small homogenous solution, reaching femtomolar detection sensitivity. This method of PLA is performed in three steps, with no wash step required.

What is enzymatic ligation?

This enzymatic ligation joins the 3’ end of the first probe with the 5’ end of the second probe, resulting in the formation of a unique target DNA reporter strand (molecule) that is a surrogate marker for the specific protein to be detected, and which contains specific molecular barcodes.