What is pegfp-c1?

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What is pegfp-c1?

pEGFP-C1encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized for brighter fluorescence and higher expression in mammalian cells. (Excitation maximum = 488 nm;emission maximum = 507 nm.) pEGFP-C1 encodes the GFPmut1 variant (4) which contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr.

How do you get eGFP to work with pegfp?

The target gene should be cloned into pEGFP-C1 so that it is in frame with the EGFP coding sequences, with no intervening in-frame stop codons. The recombinant EGFP vector can be transfected into mammalian cells using any standard transfection method.

How many silent base changes are there in eGFP?

The coding sequence of the EGFP gene contains more than 190 silent base changes which correspond to human codon-usage preferences (5). Sequences flanking EGFP have been converted to a Kozak consensus translation initiation site (6) to further increase the translation efficiency in eukaryotic cells.

By Architecture

What is pEGFP C1?

Description. pEGFP-C1 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized for brighter fluorescence and higher expression in mammalian cells. (Excitation maximum = 488 nm; emission maximum = 507 nm.)

What is pcdna3?

This pcDNA™3.1(+) vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Geneticin® selectable marker and a forward-orientation multiple cloning site. The pcDNA™3.1 Expression Vector Family.

What is pEGFP N1?

pEGFP-N1 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized for brighter fluorescence and higher expression in mammalian cells. (Excitation maximum = 488 nm; emission maximum = 507 nm.)

What is enhanced GFP?

Enhanced GFP (EGFP) is a GFP mutant with brighter fluorescence that makes the detection much more sensitive. Use of GFP as a reporter gene offers a number of advantages. These include real-time analysis, minimal sample handing, the possibility of large-quantity analysis, and high sensitivity.

What does YFP mean?

Yellow fluorescent protein (YFP) is a genetic mutant of green fluorescent protein (GFP) originally derived from the jellyfish Aequorea victoria.

What is the difference between EGFP and EGFP?

The main difference between GFP and EGFP is that the GFP (stands for Green Fluorescent Protein) is a protein that exhibits bright green fluorescence when exposed to blue light whereas the EGFP (stands for Enhanced Green Fluorescence Protein) exhibits stronger fluorescence than GFP.

What does pcDNA stand for?

Noun. pcDNA (plural pcDNAs) (genetics) plasmid cloning DNA.