What is hydroxyl radical scavenging activity?
Hydroxyl radical scavenging This assay shows the abilities of the extract and standard mannitol to inhibit hydroxyl radical-mediated deoxyribose degradation in an Fe3+-EDTA-ascorbic acid and H2O2 reaction mixture.
What is meant by radical scavenging activity?
A substance, such as an antioxidant, that helps protect cells from the damage caused by free radicals.
How do you identify antioxidant activity?
Antioxidant activity is determined as an increase in absorbance at 593 nm, and the results are expressed as micromolar equivalents of Fe2+ or in relation to a standard antioxidant . Unlike other SET-based methods, the FRAP test is carried out in acidic pH conditions (pH = 3.6)to maintain iron solubility.
What do hydroxyl radicals do?
Hydroxyl radicals are highly reactive species that attack most of the organic molecules. They are highly oxidizing in nature which is attributed to their oxidation potential (Tchobanoglous et al., 2003).
How is DPPH IC50 calculated?
you can calculate the IC50 as follows: IC50 = (0.5 – b)/a. You replace (y) by 50 in the equation y = 1.3534 x + 0.58 and you deduce ( x) .
What is a FRAP assay?
FRAP assay stands for Ferric Reducing Antioxidant Power Assay. The FRAP assay is high-throughput, adaptable and can detect antioxidant capacities as low as 0.2 mM Fe2+ equivalents.
How many types of antioxidant assays are there?
There are two general types of assays widely used for different antioxidant studies.
What produces hydroxyl radical?
Most notably the hydroxyl radicals are produced from the decomposition of hydroperoxides (ROOH) or, in atmospheric chemistry, by the reaction of excited atomic oxygen with water.
How is a hydroxyl radical formed?
The hydroxyl radical (·OH) is a powerful oxidant that is produced in a wide range of environments via the Fenton reaction (Fe2+ + H2O2 → Fe3+ + ·OH + OH-). The reactants are formed from the reduction of Fe3+ and O2, which may be promoted by organic reductants, such as hydroquinones.