What is GMR Gal4?
The GMR-GAL4 driver, which expresses the yeast transcription factor GAL4 under the control of glass multiple reporter (GMR) promoter elements, has been commonly utilized to express target transgenes, specifically in the developing eye.
Why Drosophila is suitable for the study of genetic variation?
Video Solution: Drosophila melanogaster is found to be very suitable for genetic studies because (i) They could be grown in simple synthetic medium in the laboratory (ii) They complete their life-cycle in about 14 days (iii) A single mating could produce a large number of progents (iv) Male and females are clearly …
What is ELAV GAL4?
Likewise, elav-Gal4 lines, which are generally used as postmitotic neuronal driver lines, show expression in neural progenitor cells and nearly all embryonic glial cells. Thus, in the embryo, elav can no longer be considered an exclusive marker or driver for postmitotic neurons.
What is UAS in genetics?
An upstream activating sequence or upstream activation sequence (UAS) is a cis-acting regulatory sequence. It is distinct from the promoter and increases the expression of a neighbouring gene.
Why would a geneticist study a yeast cell or a fruit fly or a mouse in order to understand human genes and human biology?
Why would a geneticist study a yeast cell or a fruit fly or a mouse in order to understand human genes and human biology? Scientists study model organisms like yeast and fruit flies in order to understand universal biochemical pathways.
What does the white gene do in Drosophila?
The white (w +) gene in Drosophila, discovered in 1910 by Thomas Hunt Morgan16, encodes a subunit of an ATP-binding cassette (ABC) transporter, which loads up pigment granules and deposits the content to pigment cells in the compound eyes, ocelli, Malpighian tubules and testis17, 18.
How many chromosomes do Drosophila have?
While drosophila only have a total of 4 chromosomes, they too display sexual dimorphism, with females carrying the double X chromosomes and males carrying XY. The two X chromosomes in female fruit flies, as in mammals, make them a homozygous sex as compared with the XY condition in males, known as heterozygous.
What is ELAV gene?
ELAV is one of a class of proteins that binds to mRNA and presumably has a regulatory function. ELAV is required for correct differentiation and maintenance of the nervous system. The gene encodes an RNA binding protein expressed in all neurons after birth, qualifying elav as a pan-neural gene.
What is the gene-switch system in Drosophila?
One inducible gene-expression system reported to allow temporal and tissue-specific control of gene expression in Drosophila is the Gene-Switch system. However, this system has not been extensively characterized in the context of aging research.
What is an example of a genetic switch?
A particularly striking example of a complex, multicomponent genetic switch is that controlling the transcription of the Drosophila even-skipped (eve) gene, whose expression plays an important part in the development of the Drosophila embryo.
What is the molecular basis of the gene switch?
The simple logic of this genetic switch first attracted the attention of biologists over 50 years ago. As explained above, the molecular basis of the switch was uncovered by a combination of genetics and biochemistry, providing the first insight into how gene expression is controlled.
Are genetic switches used to control gene expression in higher organisms?
Although the same basicstrategies are used to control gene expression in higher organisms, the genetic switches that are used are usually much more complex. Regulation of Transcription in Eucaryotic Cells Is Complex