What are Antimicrotubule agents?
A type of drug that blocks cell growth by stopping mitosis (cell division). Antimicrotubule agents interfere with microtubules (cellular structures that help move chromosomes during mitosis). They are used to treat cancer.
What is taxol used for in medicine?
Paclitaxel is used to treat various types of cancer. It is a cancer chemotherapy drug that works by slowing or stopping cancer cell growth.
Who makes paclitaxel?
Paclitaxel (sold under trade name Taxol® by Bristol-Myers Squibb) is a very effective plant-derived chemotherapy drug used to treat breast, ovarian, lung, bladder, prostate, melanoma, esophageal, as well as other types of solid tumor cancers.
What is carboplatin and paclitaxel?
Paclitaxel and carboplatin are the names of a chemotherapy combination. It’s also known as PC and CarboTaxo. It is a treatment for a number of different cancer types.
What is tubulin cytoskeleton?
Microtubules are polymers of tubulin that form part of the cytoskeleton and provide structure and shape to eukaryotic cells. Microtubules can grow as long as 50 micrometres and are highly dynamic. The outer diameter of a microtubule is between 23 and 27 nm while the inner diameter is between 11 and 15 nm.
What is alpha and beta tubulin?
Definition. Alpha tubulin refers to one of the two protein components of a microtubule attached to GTP while beta tubulin is the second protein component of a microtubule. Thus, this is the fundamental difference between alpha and beta tubulin.
What is aphidicolin block?
Aphidicolin is a reversible inhibitor of eukaryotic nuclear DNA replication. It blocks the cell cycle at early S phase. It is a specific inhibitor of DNA polymerase Alpha and Delta in eukaryotic cells and in some viruses (vaccinia and herpesviruses) and an apoptosis inducer in HeLa cells.
How do you use nocodazole?
For mitotic cell arrest, add nocodazole to a final concentration of 50 ng/mL (8 pm). Prepare a stock solution by dissolving nocodazole powder in DMSO (e.g. 5 mg/mL) and store frozen at -20 °C. Incubate cells with nocodazole for no longer than 10 – 11 h at 37 °C in a humidified atmosphere with 5% CO2.
How do antitubulin agents block cell division on microtubules?
Results and conclusion: Although it is widely accepted that antitubulin agents block cell division by inhibition of the mitotic spindle, the mechanism of action of antitubulin agents on microtubules remains to be determined.
Does PGP mediate multidrug resistance to antitubulin?
Although the multidrug resistance phenotype mediated by Pgp appears to be an important mechanism of resistance to these agents, alterations of microtubule structure resulting in altered microtubule dynamics and/or altered binding of antitubulin agents may constitute a significant mechanism of drug resistance. Research Support, Non-U.S. Gov’t
How many compounds are effective inhibitors of tubulin polymerization?
In summary, 20 compounds (representing 11 distinct chemical species) were effective inhibitors of tubulin polymerization and caused the accumulation of cells arrested in mitosis in tissue culture. These were all synthetic compounds with relatively simple chemical structures, and they all interfered with the binding of colchicine to tubulin.
Can vinca alkaloids and taxanes act synergistically as antitumor agents?
These data suggest that vinca alkaloids and taxanes may act synergistically as antitumor agents and may be administered as combination chemotherapy in the clinic.