How much does agar plates cost?

How much does agar plates cost?

Catalog No. Plate Type Price/Plate
4001-100 LB Agar Plates, 100x15mm $1.50
4001-150 LB Agar Plates, 150x15mm $2.50
4002-100 LB Agar w/ Ampcillin 100ug/ml Plates*, 100x15mm $1.50
4002-150 LB Agar w/ Ampcillin 100ug/ml Plates*, 150x15mm $2.50

How do you make an NGM plate?

Following is the protocol to prepare NGM plates:

  1. Weigh 15 g agar, 2.4 g NaCl, 2 g Tryptone, and 2.72 g KH2PO4 in 2 L conical flask, make volume up to 1 L with water, autoclave and allow it to cool to 60° C in a water bath.
  2. Add 0.8 ml each of 1 M CaCl2, cholesterol (5 mg/ml in ethanol), and 1 M MgSO4.

How do you seed NGM plates?

To seed large (9cm) NGM plates: drop 5 drops OP50 in the middle of a plate. Use a sterile pipette at a very shallow angle to spread the OP50 liquid around so that a thin pool of liquid covers the middle 2/3 of the plate. Set the seeded plates at 37° overnight, or at room temp. for 2 days.

How do you pick a worm off an NGM agar plate?

4.1. Transferring worms grown on NGM plates

  1. C.
  2. Several methods are used for transferring C.
  3. To pick a worm identified under the dissecting microscope, slowly lower the tip of the wire and gently swipe the tip at the side of the worm and lift up.

How long do agar plates last?

Most plates are OK for a month or 2 if wrapped and refrigerated. They may be OK (we used to keep Brilliant Green Plates for about 3 months), but its probably easiest to make new ones rather than go through some rigmarole of testing.

What is the difference between an agar plate and a Petri dish?

Agar is a gelatinous polymer substance derived from red algae and commonly used in a biological laboratory setting as a substrate. Agar plates are petri dishes containing agar in combination with a growth medium to culture microorganisms such as bacteria.

What is NGM agar?

C. elegans is maintained in the laboratory on Nematode Growth Medium (NGM) agar which has been aseptically poured into petri plates. Sometimes they can also be grown on LB-NGM, where the LB in the media will promote better growth of the bacteria the worms feed on.

Can you autoclave cholesterol?

For this reason, we add cholesterol after filtration or autoclaving of media for NGMA plates. For NGM/HGM plates: Use the unfiltered but sterile 5 mg/mL cholesterol in ethanol. To make this cholesterol, Take an autoclaved sterile 250 mL bottle.

Can E coli grow at 4 degrees?

So, yes — E. coli does grow at 4 degrees, and you shouldn’t store your transformation plates directly, rather you should pick your transformants to a fresh plate and store that.

Can C. elegans be frozen and revived?

Healthy cultures of C. elegans can be frozen and then defrosted and revived when needed.

Why are agar plates stored upside down?

Agar plates are used to grow microorganisms in the lab. The plates are often stored in the refrigerator, which can cause condensation on the lid. Agar plates should be kept inverted whenever possible to prevent water from dripping onto the agar surface.

How do you preserve agar plates?

Store plates upside down in a refrigerator or cold room. If they are stored in a room, check the plates after a few hours for condensation in the lid. If you have the plates upside down and there is condensation in the lid, there must be some heat source above that is driving water out of the agar and into the lid.